Fig. 7
From: A novel luciferase-based assay for quantifying coronavirus-induced syncytia
SARS-CoV-2 spike protein-induced cell fusion. (a) Visualization of SARS-CoV-2 spike expression-induced syncytia in parental Vero cells. Syncytia are indicated by a dashed line in light microscopy images captured 24 hpt. Scale bar: 100 μm. (b) Upregulation of split-Gluc expression in parental Vero cell culture media. Split-Gluc activity was determined at 24 hpt in co-cultured cells transiently expressing SARS-CoV-2 S and ssTau-Gn or ssTau-Gc. (c) Enhancement of SARS-CoV-2 spike-mediated syncytial formation by human and feline ACE2 in Fcwf-4-stable Tau cells. Syncytia are indicated by a dashed line in light microscopy images obtained 24 hpt. Scale bar: 100 μm. (d) ssTau-split Gluc activity of the Fcwf-stable Tau cells shown in panel (c). The empty control (Vect) or SARS-CoV-2 S expression vectors were co-transfected with human or feline ACE2 expression vectors. Split-Gluc activity was determined at 24 hpt.
