Fig. 3
Antioxidant, inflammation and hypoxia linked genes and proteins expression at 24h and 28 days in the FC. (A) Relative gene expression of Sod1, Sod2, Catalase, IL-1β and Mcp1 in the FC at 24h post-exposure ± SEM. Arbitrary units (a.u.). n = 7–8 per group. (B) Relative gene expression of Sod1, Sod2, Catalase, IL-1β and Mcp1 in the FC at 28 days post-exposure ± SEM. Arbitrary units (a.u.). n = 7–8 per group. (C) FC 24h post-exposure protein expression of NRF2 and HIF1ɑ, and pellet/supernatant ratio for NRF2 to assess nuclear translocation of NRF2 ± SEM. Arbitrary units (a.u.). n = 5–6 per group. (D) FC 28 days post-exposure protein expression of NRF2 and HIF1ɑ, and pellet/supernatant ratio for NRF2 to assess nuclear translocation of NRF2 ± SEM. Arbitrary units (a.u.). n = 5–6 per group. (E, F, G) Representative Western blot membranes used to quantify the protein expression of NRF2 and normalize with GAPDH (whole cell lysate or cytosolic fraction) or β-actin (nuclear fraction). (H) Representative Western Blot membrane for HIF1ɑ protein expression quantification. Western blot membranes images were cropped (Figure 3E to H) and original blots are presented in Supplementary Figure 1 (Sup. Figure 1E to H). Western blots were done in duplicates. All results passed normality test and were analyzed using a One-way ANOVA, except IL-1β which did not pass normality tests and was analyzed using a Kruskal–Wallis test. All groups were compared to each other within each time point. Significant differences between groups are indicated by asterisks (*p < 0.05; **p < 0.01).
