Fig. 4
From: Inhibition of CDK5 signaling mediated inflammation in macrophages promotes cutaneous wound healing
Inhibition of CDK5 activity promotes wound healing and M2 phenotype transformation of macrophages. (A) Schematic diagram of the intervention with Roscovitine on the full-thickness cutaneous model on the dorsal skin of mice; (B) Representative images of full-thickness cutaneous wound models at different time points. Scale bar = 0.2 cm. Lower panel: quantitative analysis of wound closure percentage (n = 5). *P < 0.05; **P < 0.01. (C) Dual immunofluorescence staining to observe the in situ expression of IL-1β (red) and IL-10 (green) at the wound bed on day 5. Scale bar = 50 μm. Right panel: the quantitative analysis (n = 5). **P < 0.01. (D) Dual immunofluorescence staining to observe the in situ expression of F4/80 (green) with IL-1β (red) or IL-10 (red) in day 5 wound tissues. Scale bar = 50 μm. Right panel: the percentage of iNOS + F4/80-positive cells and CD163 + F4/80-positive cells (n = 5). **P < 0.01.
