Fig. 3 | Scientific Reports

Fig. 3

From: Comparison of the effects of fractional microneedle radiofrequency and microneedling on modulating the senescent fibroblast milieu in aged skin

Fig. 3

Changes in the cellular milieu in the dermis following MNRF treatment. (a) Quantification of the total cell number in the dermis after MN and MNRF treatments. (b) Representative images of immunofluorescence staining for the fibroblast marker, HSP47 (red), and DAPI (blue) in the skin treated with MN and MNRF. The graph on the right indicates the number of HSP47-positive dermal fibroblasts after MN and MNRF treatments (n = 25). (c) Representative images of immunofluorescence staining for the leukocyte marker, CD45 (green); and macrophage marker, CD68 (red); and DAPI (blue) in skin treated with MN and MNRF. The graphs on the right represent the number of CD45-positive leukocytes and CD68-positive macrophages in the dermis after MN and MNRF treatments (n = 25). Original magnification ×400. Statistical significance: **p < 0.01, ns indicates no significance.

Back to article page