Fig. 4 | Scientific Reports

Fig. 4

From: Comparison of the effects of fractional microneedle radiofrequency and microneedling on modulating the senescent fibroblast milieu in aged skin

Fig. 4

Repopulation of senescent fibroblasts by non-senescent fibroblasts after MNRF treatment. (a) Representative images of immunofluorescence staining for the senescence marker, p16INK4A, and fibroblast marker, HSP47, after MN and MNRF treatments. The graphs on the right represent the results of the quantitative assessment of the (b) percentage of p16INK4A-positive cells among HSP47-positive dermal fibroblasts, (c) number of p16INK4A- and HSP47- double positive dermal fibroblasts, (d) p16INK4A-positive dermal cells, (e) p16INK4A-positive epidermal cells, and (f) p16INK4A-negative HSP47-positive dermal fibroblasts in the skin treated with MN and MNRF (n = 25). Original magnification ×400. Statistical significance: ***p < 0.001, ****p < 0.0001. ns indicates no significance.

Back to article page