Fig. 4 | Scientific Reports

Fig. 4

From: Integrated 3D imaging of FFPE lung tissue combining microCT, light and electron microscopy allows for contextualized ultrastructural and histological analysis

Fig. 4

LM and SRµCT hybrid depiction of BADJ. (A) Exemplary slice from the reconstructed synchrotron radiation µCT data set of the tissue cylinder acquired via punch biopsy, (B) Digital light microscopy image (LM), AB-PAS stain. (C) Volume rendering shows the cylindrical shape of the SRµCT data set. (D) Hybrid image of the registered LM and the SRµCT data set. (E) A magnified portion of the hybrid image shows a BADJ. The black arrows in the magnified LM insert indicate the alveolar septal tips (fiber content), while in the CT data the complex 3D architecture of the axial connective tissue system is portrayed. A carina-like main cable element (solid line) divides the BADJ in two primary alveolar ducts. The complexly structured helical line element gives rise to alveolar entrance rings (dashed lines).

Back to article page