Fig. 7 | Scientific Reports

Fig. 7

From: Integrated 3D imaging of FFPE lung tissue combining microCT, light and electron microscopy allows for contextualized ultrastructural and histological analysis

Fig. 7

Hybrid image, created after elastic registration and subsequent integration of SRµCT, histology and SEM. (A) Overview of all imaging modalities (SRµCT (grey), H&E stained light microscopy (h) and same-slide SEM (s)) of the workflow combined into one. A bronchiolus (b1, blue) with surrounding cartilage (*, pink), a venous blood vessel (v, red) and a terminal bronchiolus with its bronchoalveolar ductus (b2) and associated alveoli have been segmented from the microCT data sets. (B) Zoomed in view of the hybrid image. Regions of interest for SEM (s), in this example containing septal tips, had been selected within the light microscopy image.

Back to article page