Fig. 2 | Scientific Reports

Fig. 2

From: GliaTrap is a biodegradable, non-swelling and non-inflammatory hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells

Fig. 2

CXCL12 induces GSC chemotaxis in a biomimetic device. (A) Design of the negative mold for the biomimetic device and development of the PDMS device. (B) Time-lapse images between T = 0 and T = 42 h for dextran release from our hyaluronic acid/collagen II-based (HA/Col) hydrogel in the device using Zen 2.5D visualization software (Zeiss). (C) Time-lapse images of GSC migration in the device in the presence or absence of GliaTrap + CXCL12. Images were acquired each day for 4 consecutive days. GSC spheroids were embedded in the middle chamber of the device in Collagen I. (D) Coordinates of each migrating GSC in control or GliaTrap devices at day 4 were plotted using R. (E) Quantification of the ratio of migrating GSCs between the left and right side of the middle line of each GSC spheroid. GliaTrap induces significant increase in the number of GSCs migrating towards the GliaTrap containing chamber of the device (*p < 0.05, Student’s t-test). (F) Coordinate single cell migration data were used to calculate the polar coordinates and degrees from the origin and visualized as a rose plot using the R package ggplot2.

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