Fig. 1

Polyunsaturated fatty acid (PUFA) composition in various tissues following dietary oil feeding. The ratio of omega-3 to omega-6 PUFA was determined in serum (A), follicular fluid (B), skeletal (trapezius) muscle tissue (C), and granulosa cells (D) prior to (BL) and following 6 weeks of corn (N6) or flaxseed (N3) diets, and following 6 additional weeks with the added dietary supplement (+ DS). Follicular fluid and granulosa cells were collected from preovulatory dominant follicles 24 ± 2 h after maturation induction and skeletal muscle biopsies were obtained from the trapezius muscle. Graphs represent mean ± SEM of N = 6 mares per group. Superscripts differ at P < 0.05 between weeks within mare’s supplementation group by Tukey’s HSD test. Noted P values indicate significant differences in the combined 6 wk and + DS time points between N6 and N3 cohorts by independent sample t-tests at P < 0.05.