Fig. 2
Platelet oxidative metabolism. High resolution respirometry was performed on platelets isolated from mares at baseline (BL), following 6 weeks of feeding the flaxseed (N3) or corn oil (N6) supplements, and following an additional 6 weeks with dietary supplement (+ DS). Rates of oxygen consumption (JO2) were measured in intact cells under basal condition supported by endogenous substrates (A), followed by permeabilization with digitonin and the addition of malate and palmitoylcarnitine to generate the LEAK JO2 (B). ADP was then added to generate the OXPHOS supported by fatty acid oxidation (FAOx; C) and enable calculation of FAOx-linked coupling control (D), followed by the addition of pyruvate, glutamate and succinate to support maximal OXPHOS-linked JO2 (E). Graphs represent mean ± SEM of N = 6 mares per group. Superscripts differ at P < 0.05 between weeks within mare’s supplementation group by Fisher’s LSD test following a significant ANOVA.
