Fig. 5
Mitochondrial analysis pipeline reveals pathological differences between wild type and mdx mice. (A) Schematic figure of mitochondrial segmentation and morphological quantification from TEM images of the quadriceps muscle in wild type and mdx mice. Left are representative TEM images of mitochondria from wild-type and mdx mice. Right are corresponding results from mitochondrial segmentation and morphological quantification (Log-transformed). (B) Box and whiskers plot showing morphological differences in mitochondria between wild-type and mdx mice. Mdx mice exhibit significantly altered mitochondrial features compared to wild-type mice (mean ± SD, p = 2.2 × 10−16, independent two-sample t-test. N = 18–27). Statistical significance is indicated by asterisks. To accommodate values below 1 and to prevent negative outputs during log transformation, we added a constant offset of 1 to all measurements before applying the log transformation.
