Fig. 5
TIMM8A knockdown inhibited the of breast cancer in vitro. (A, B) Knockdown efficiency of TIMM8A via siRNA transfection analyzed proliferation by qRT-PCR and Western blot. (C, D) Proliferative capacity of MCF7 and MDA-MB-231 cells after knockdown of TIMM8A assayed by CCK8. (E–G) Clonal formation ability of TIMM8A-knockdown MCF7 and MDA-MB-231 cells. Clonal formation rate = effective clones/plating cell numbers × 100%. (H-J) Cell cycle profiles of TIMM8A-knockdown MCF7 and MDA-MB-231 cells generated by flow cytometry using PI staining. The results shown are representative of at least three independent experiments. The student’s t-test or one-way ANOVA was used to detect the differences among groups. *P < 0.05, ***P < 0.001. Samples were derived from the same experiment and gels/blots were processed in parallel. Original blots/gels are presented in the Supplementary File.
