Table 4 Prevalence of reactivity of HCV by chemiluminescence (CLIA) and nucleic acid testing (NAT).
HCV (N/%) | Total (N = 87620) | Assuit (N = 75156) | Mansoura (N = 12464) | |||
|---|---|---|---|---|---|---|
CLIA | NAT | CLIA | NAT | CLIA | NAT | |
Non-reactive | 86,143/98.31 | 86,292/98.48 | 73,858/98.27 | 73,988/98.45 | 12,285/98.56 | 12,304/98.72 |
Reactive | 1477/1.69 | 1328/1.52 | 1298/1.73 | 1168/1.55 | 179/1.44 | 160/1.28 |
Anti-HCV + ve NAT + ve (TP) | 1305/1.49 | 1149/1.53 | 156/1.25 | |||
Anti-HCV + ve NAT-ve (FP) | 172/0.2 | 149/0.2 | 23/0.19 | |||
Anti-HCV -ve NAT + ve (FN) (NAT yield) | 23/0.03 | 19/0.03 | 4/0.03 | |||
Anti-HCV -ve NAT-ve (TN) | 86,120/98.28 | 73,839/98.24 | 12,281/98.53 | |||
Sensitivity, Specificity, PPV, NPV and accuracy of chemiluminescence compared to gold standard test NAT (value, 95% CI) | ||||||
Sensitivity | 98.27% (97.41–98.90%) | 98.37% (97.47–99.02%) | 97.50% (93.72–99.31%) | |||
Specificity | 99.80% (99.77–99.83%) | 99.80% (99.76–99.83%) | 99.81% (99.72–99.88%) | |||
PPV | 88.35% (86.73–89.81%) | 88.52% (86.79–90.05%) | 87.15% (81.84–91.08%) | |||
NPV | 99.97% (99.96–99.98%) | 99.97% (99.96–99.98%) | 99.97% (99.91–99.99%) | |||
Accuracy | 99.78% (99.74–99.81%) | 99.78% (99.74–99.81%) | 99.78% (99.68–99.86%) | |||
Inter-rater agreement - % - Kappa coefficient value | 99.77% 0.929 (very good) | 99.77% 0.930 (very good) | 99.78% 0.919 (very good) | |||
