Fig. 7
Validation of high-risk gene expression in colon adenocarcinoma. (A,C,E,G) Immunohistochemistry (IHC) images from the HPA database showing the expression of MAP3K7, HEXIM1, HCFC2, and DICER1 in normal and tumor tissues. The tumor tissues exhibit significantly higher staining intensity, indicating elevated expression of these genes. Scale bar = 100 µm. (B,D,F,H) Quantification of IHC staining using ImageJ software reveals significantly higher average optical density (AOD) of MAP3K7, HEXIM1, HCFC2, and DICER1 in tumor tissues compared to normal tissues. (I) Western blot (WB) analysis confirms the overexpression of these genes in tumor tissues compared to normal tissues, with GAPDH used as a loading control. (J) Quantification of WB results shows the relative expression levels of MAP3K7, HEXIM1, HCFC2, and DICER1 in colon adenocarcinoma, with band intensities measured using ImageJ and normalized to GAPDH. The tumor tissues exhibit significantly higher protein expression compared to normal tissues.
