Fig. 3
From: Role of galectin-9 in the development of gestational diabetes mellitus
Expression of Gal-9 in placenta derived from B6-Lgals9tm1glp (KO) and wild type (WT) pregnant mice. (A) Immunohistochemical staining of Gal-9 in placenta at gestational day (GD19). Gal-9 is observed in the cytoplasm and cell surface of trophoblast giant cells (TGC, red arrows) and spongiotrophoblast cells (SP, yellow arrows) in SFD WT, SFD KO, HFD WT, and HFD KO mice. D, decidua; JZ, junctional zone; Lab, labyrinth. B. Western blot analysis of Gal-9 in placenta at GD19 and densitometric analyses (n = 3 in each group). C. mRNA expression of Lgals9 normalized by Rplp0 and Gapdh in placenta, liver, and ovarian fat derived from SFD WT (n = 6), SFD KO (n = 5), HFD WT (n = 5), and HFD KO (n = 6). Data are shown as mean ± SD and analyzed by one-way ANOVA with Tukey test (*p < 0.05; **p < 0.01). SFD, standard fat diet, 10% fat; HFD, high fat diet, 60% fat.
