Fig. 4
From: Role of galectin-9 in the development of gestational diabetes mellitus
Proliferating cell nuclear antigen (PCNA) and apoptosis assay in placenta derived from B6-Lgals9tm1glp (KO) and wild type (WT) pregnant mice. (A) Immunohistochemical staining of PCNA in placenta at gestational day 19 (GD19). (B) The counts of PCNA positive cells in whole placenta sections in SFD WT (n = 3), SFD KO (n = 3), HFD WT (n = 3), and HFD KO (n = 4), and mRNA expression of Pcna normalized by Rplp0 in SFD WT (n = 5), SFD KO (n = 5), HFD WT (n = 5), and HFD KO (n = 6). (C) Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL assay) of placenta at GD19. The apoptotic cells are labeled by 3,3′-Diaminobenzidine (DAB). SFD, standard fat diet, 10% fat; HFD, high fat diet, 60% fat.
