Table 1 Optimization of polysaccharides production by Lactiplantibacillus plantarum HAN99 using the Plackett Burman experimental design.

Trial	Factors under study	Independent factors												EPS (mg/ml)
Trial	Factors under study	RS	IS	IG	CV	IT	P	Y	G	T	K₂H	SC	Mg	EPS (mg/ml)
1	RS IT G T K₂H SC	+	-	-	-	+	-	-	+	+	+	+	-	40
2	RS IS P T Mg	+	+	-	-	-	+	-	-	+	-	-	+	50
3	RS IS IG Y SC	+	+	+	-	-	-	+	-	-	-	+	-	70
4	RS IS IG CV G SC Mg	+	+	+	+	-	-	-	+	-	-	+	+	37.6
5	IS IG CV IT T K₂H	-	+	+	+	+	-	-	-	+	+	-	+	46.6
6	RS IG CV IT K₂H	+	-	+	+	+	+	-	-	-	+	-	-	110
7	IS CV IT P Y K₂H SC	-	+	-	+	+	+	+	-	-	+	+	-	10
8	RS IG IT P Y G K₂H Mg	+	-	+	-	+	+	+	+	-	+	-	+	66.70
9	RS IS CV P Y G T	+	+	-	+	-	+	+	+	+	-	-	-	26.67
10	IS IG IT Y G T K₂H	-	+	+	-	+	-	+	+	+	+	-	-	40
11	IG CV P G T SC	-	-	+	+	-	+	-	+	+	-	+	-	15
12	RS CV IT Y T K₂H SC Mg	+	-	-	+	+	-	+	-	+	+	+	+	140
13	IS IT P G K₂H SC Mg	-	+	-	-	+	+	-	+	-	+	+	+	3.30
14	IG P Y T SC Mg	-	-	+	-	-	+	+	-	+	-	+	+	20
15	CV Y G Mg	-	-	-	+	-	-	+	+	-	-	-	+	190
16	---------------------------	-	-	-	-	-	-	-	-	-	-	-	-	50

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