Table 5 Comparison of detection results.
From: Artificial intelligence enhanced electrochemical immunoassay for staphylococcal enterotoxin B
Refernces | Method | Technique | \(R^2\) | Concentration | LOD | RSD | Time | Sample | Application |
|---|---|---|---|---|---|---|---|---|---|
Range | Consumed | Volume | Scenario | ||||||
Immuno- | Colloidal gold | – | 10–1000 ng/mL | 10 ng/mL | – | A few min | 100 μL | Rapid | |
Chromatographic | Labeling | Screening | |||||||
Test strip | |||||||||
LFA | Colloidal gold | – | 1–1000 ng/mL | 10 ng/mL | – | 10 min | 100 μL | Food | |
Labeling, | |||||||||
Double antibody | Testing | ||||||||
Sandwich | |||||||||
ELISA | – | 0.9824– | 0–20 ng/mL | 1–5 ng/mL | – | 8-10 h | – | – | |
0.998 | SEs | ||||||||
ELISA | Sandwich | 0.9935 | 1–512 ng/mL | 0.3 ng/mL | – | – | 100 μL | – | |
Nbs-ELISA | |||||||||
Sliding microtubes | Sandwich | – | 0–100 ng/mL | 0.5 ng/mL | – | 30 min | – | Laboratory | |
Immunoassay | Chip | ||||||||
ELISA | Sandwich | – | 0.25–100 ng/mL | 0.5–0.75 ng/mL | – | A few hours | 100 μL | Meat | |
Immunoassay | SEA | ||||||||
Electrochemical | GR-Ch-AuNPs | – | 10 ng/mL | 5 ng/mL | – | 35 min | – | On-site | |
Sandwich | Modified | − 10 μg/mL | Detection | ||||||
Immunoassay | Electrode | ||||||||
Fluorescence | Carbon Dot | 0.9849 | 0.5–10 ng/mL | 0.5 ng/mL | – | 15 min | 50 μL | Food | |
Resonance | Acriflavine | ||||||||
energy Transfer | Nano-assembly | Testing | |||||||
(FRET) | Detection | ||||||||
Electrochemical | Platinum | 0.989 | 1 ng/mL | 1 ng/mL | 3-8.8% | – | – | – | |
Nanoparticle | |||||||||
Modification, | |||||||||
Immunosensor | Hydrogen evolution | − 1 μg/mL | |||||||
Suppression method, | |||||||||
Indirect sandwich | |||||||||
ELISA | |||||||||
ELISA | Sandwich | – | – | 0.5 ng/mL | – | 45 min | 1 mL | Food | |
Immunoassay | Testing | ||||||||
ELISA | Sandwich | – | 0.1 ng/mL | 1 ng/mL | – | A few hours | 100 μL | Food safety, | |
Immunoassay | − 1 μ/mL | Clinical diagnosis | |||||||
SEG | |||||||||
Biosensor | Electroosmotic | – | 5–100 ng/mL | 5 ng/mL | 11.50% | A few min | – | – | |
filtration | |||||||||
Biosensor | Surface plasmon | 0.99 | 1–1000 ng/mL | 10 ng/mL | – | 5–8 min | 40 μL | Food | |
resonance (SPR) | contamination | ||||||||
This work | Electrochemical | Machine learning | 0.998 | 1 ng/mL–10 μg/mL | 1 ng/mL | 3.62–9.21% | 2 min | 20 μL | Multiple |
Immunosensor | Application | ||||||||
Scenarios |
