Fig. 6
The influence of drug combinations on the PI3K/AKT/mTOR pathway, B-catenin, GSK3B, BCL2, and Hsp90 in Huh-7 liver cancer cells was examined. RT-qPCR was employed to quantify mRNA fold changes for genes within these pathways, specifically PI3K, AKT, mTOR, B-catenin, GSK3B, BCL2, and Hsp90. In Huh-7 cells, both palbociclib and the C + P combination significantly decreased the expression of Hsp90, GSK3B, and PI3K/AKT/mTOR pathway genes, relative to cisplatin monotherapy and the untreated control. Conversely, cisplatin monotherapy significantly increased the expression of these genes compared to palbociclib, the C + P combination, and the control. All three treatments (palbociclib, cisplatin, and C + P) significantly reduced the expression of oncogenic BCL2 and B-catenin relative to the control. ****p < 0.0001, with additional significant p values detailed in the figure, as determined by one-way ANOVA with Tukey’s post hoc test.
