Fig. 1

Mapping of TLR7 expression across URT and LRT. Nasal, BAL and lung tissues from uninfected WT C57Bl/6 mice were analyzed. (A) Gene expression of pattern recognition receptors was measured by RT-qPCR and presented relative to RPS18 housekeeping (2^−ΔCt). (B) Intracellular staining of TLR7 was determined by flow cytometry with positively stained cells gated above unstained isotype controls. The mean fluorescence intensity (MFI) for each tissue is also presented. The proportion of TLR7 expressing (TLR7+) cells staining for (C) CD45 or (D) other surface markers (gated on CD45 + cells) to identify immune cell types was determined. Data is expressed as mean ± SEM, n = 4–5 biological replicates. Statistical analysis was conducted using one-way ANOVA test followed by Tukey’s post hoc test for multiple comparisons (***p < 0.001, ****p < 0.0001).