Fig. 3

Flow cytometry analysis of LRS chamber cell products revealed differences in CD4⁺ and CD8⁺ T cell frequencies. Leukocyte product was recovered from LRS chamber and stained for flow cytometry analysis. Frequencies of cell populations were determined by manual gating, including CD3⁺ CD4⁺ T cells (a), CD3⁺ CD8⁺ T cells (b), CD45⁺ leukocytes (c), CD3⁺ T cells (d), CD19⁺ B cells (e), CD56⁺ NK cells (f), CD56^high NK cells (g) and CD14⁺ monocytes (h) (n = 15 for Trima 6, n = 9 for Trima 7 and n = 14 for Trima 7 PAS; statistical analyses were carried out using one-way ANOVA with Tukey test for multiple comparison; effect size is reported as Cohen’s d).