Fig. 11 | Scientific Reports

Fig. 11

From: MiR-125b-5p ameliorates ventilator-induced lung injury in rats by suppressing ferroptosis via the regulation of the Keap1/Nrf2/GPX4 signaling pathway

Fig. 11

MiR-125b-5p inhibits ferroptosis by targeting the Keap1/Nrf2/GPX4 axis, thereby alleviating VILI. (A,B) expression of miR-125b-5p and Keap1 mRNA in the lung tissue of rats in the different groups were determined by qRT-PCR. (C) Protein expression of Keap1 was detected by western blotting, with β-actin as the internal control. (D) Observation of the lung tissue with the naked eye. (E) Wet/dry weight ratio of the lung tissue. (F) Total protein content in BALF. (G) Lung tissue HE staining (200 × and 400 ×) based on observation with a light microscope and lung injury score. (**P < 0.01. ***P < 0.001. ****P < 0.0001). VILI, ventilator-induced lung injury; BALF, bronchoalveolar lavage fluid; HE, Hematoxylin and eosin; qRT-PCR, real-time quantitative polymerase chain reaction.

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