Fig. 5 | Scientific Reports

Fig. 5

From: MiR-125b-5p ameliorates ventilator-induced lung injury in rats by suppressing ferroptosis via the regulation of the Keap1/Nrf2/GPX4 signaling pathway

Fig. 5

Ferroptosis was present in MS-induced injury cell models. (A) Protein expression of SLC7A11, GPX4 and ACSL4 in ATII cells were detected by western blotting, with β-actin as the internal control. (B) ROS was detected by immunofluorescence. Green: ROS. Magnification: 40x. Content of GSH (C), MDA (D) and cell iron (E) in MS-induced injury cell model. (**P < 0.01. ***P < 0.001. ****P < 0.0001). MS, mechanical stretch; SLC7A11, Solute carrier family 7 member 11; GPX4, Glutathione peroxidase-4; ACSL4, Acyl-CoA synthetase long-chain family member 4; ROS, Reactive oxygen species; GSH, Glutathione; MDA, malondialdehyde.

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