Fig. 2

Characterisation of the effects of therapies on the tumour microenvironment. (a–k) Flow cytometry analyses of cell positive for the indicated markers in the tumour immune microenvironment expressed as percentage of tumour infiltrating CD45⁺ cells in each of the indicated therapy groups. Statistically significant differences between groups were determined using Welch’s ANOVA test followed by Dunnett’s T3 multiple comparisons test. (l–v) Immunohistochemical analyses of the tumour immune microenvironment. Densities of cells/mm² that express the indicated markers in each of the therapy groups are shown. F4/80 positivity was quantified using percentage positive pixels (%PP). N: adjacent normal tissue, T: ccRCC tumour tissue. Statistical differences between groups were determined using Welch’s ANOVA test followed by Dunnett’s T3 multiple comparisons test.