Fig. 3

Evidence for recognition of ccRCC tumour cells by peripheral T cells induced by immune checkpoint therapy. (a) Overview of the experimental workflow. (b) Representative flow cytometry histograms of CD4⁺ and CD8⁺ T cells from an ICI ctrl mouse or an ICI treated mouse, with or without activation by α-CD3/α-CD28 beads and with or without co-culture with ccRCC cells from the same mouse. (c,d) Summary of the results of the assay for all splenocyte-ccRCC cell line pairs for CD4 ⁺ (c) and CD8a ⁺ (d) T cells. Each dot represents T cells from an individual animal and shows the median fluorescence intensity (MFI) of the indicated antibody staining. Statistical differences between groups were determined using the non-parametric Mann–Whitney test.