Fig. 5

Gene Regulatory Networks of Fibroblast Subpopulations in the MI and Sham Groups. (A) The heatmap displayed the differential expression of the top five TFs in the three fibroblast subpopulations. (B) Fibroblast subpopulations were highlighted as light purple dots in the UMAP plot (middle). The ranking of regulatory factors within subpopulations based on their Regulator Specificity Scores (RSS) was shown (left). The binary Regulator Activity Scores (RAS) of the highest regulatory factors (normalized by Z-score with a cutoff of 2.5, converted to 0 or 1) were mapped as red dots on the UMAP plot (right). (C) UMAP plots illustrated the expression patterns of Hdac2, Bclaf1, Tcf3, and Meis1 across all subpopulations, excluding Tead1. (D) Violin plots compared the AUC values of five TFs (Tead1, Hdac2, Bclaf1, Tcf3, and Meis1), ranking their activity across fibroblast subpopulations. (E) The heatmap highlighted the differential expression of the top five TFs between the MI and Sham groups. (F) The MI and Sham group distributions were highlighted as light purple dots in the UMAP plot (middle). The ranking of regulatory factors in the two groups based on their RSS scores was displayed (left). Binary RAS scores of the top regulatory factors, mapped as red dots, illustrated their distribution across the UMAP plot (right). (G) Violin plots depicted the differences in AUC values for the five TFs (Tead1, Hdac2, Bclaf1, Tcf3, and Meis1) between the MI and Sham groups.