Fig. 7 | Scientific Reports

Fig. 7

From: Single-cell RNA sequencing reveals the potential role of Postn(+) fibroblasts in promoting the progression of myocardial fibrosis after myocardial infarction

Fig. 7

In Vitro Experiments on Postn Knockdown in Cardiac Fibroblasts. (A) Relative expression of Postn mRNA in cardiac fibroblasts with si-Postn knockdown. (B) Relative expression of Postn protein in cardiac fibroblasts with si-Postn knockdown. (C) CCK-8 assay demonstrated a significant decrease in cell viability following Postn knockdown. (D) Transwell migration assays showed that Postn knockdown significantly reduced the migration ability of cardiac fibroblasts. (E) Adhesion assays revealed a significant reduction in cell adhesion ability following Postn knockdown. (F) Annexin V-FITC/PI double staining flow cytometry showed that Postn knockdown significantly induced apoptosis in cardiac fibroblasts compared to the NC group.

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