Fig. 4

Doxylamine and Gemfibrozil induce cytotoxicity in GBM cells: (a) Bar graph representing dose and time kinetics of Doxylamine (Doxy), Gemfibrozil (Gem), Diprophylline (Dipro), and Valproic Acid (VPA), and compared with the Standard Drug TMZ by MTT assay, (b) Crystal violet images capturing the viable cells after 24 h, 48 h, and 72 h of drugs treatment, scale bar represents 10 μm (c) Immunoblot showing expression of proliferation marker PCNA after (i) 24 h and (ii) 48 h of incubation, (d) Cleaved Caspase 3 expression by Immunoblotting confirming apoptosis of the cells (cropped image of the blot is provided), (e) Analysis of the total number of cell death by AnnexinV/PI data assay obtained after 48 h of drug treatment. p-value ≤ 0.05 is considered significant, and * refers to a p-value less than or equal to 0.03, ** refers to a p-value less than or equal to 0.002, and *** signifies a p-value less than 0.0001 between control and different test groups. All experiments were performed in triplicate unless stated otherwise, and all data were generated using the U87 cell line, unless specified differently.