Fig. 3

Characterization of MyCAFs and ImmCAFs. (A) Results of GO enrichment analysis for ImmCAFs. The larger dot represented a higher number of enriched genes, and the redder color of the dot represented a more significant relationship. (B) Results of GO enrichment for MyCAFs. The larger dot represented a higher number of enriched genes, and the redder color of the dot represented a more significant relationship. (C) Histogram showing differences in the proportion of ImmCAFs and MyCAFs between tumor and normal tissue samples. (D) UMAP plot showing the selection of ImmCAFs as the starting point for the cell trajectory analysis. ImmCAFs were indicated by purple color and MyCAFs were indicated by orange color. (E) Progression from ImmCAFs to MyCAFs with changing proposed time for cell trajectory analysis. (F) Top altered genes during the transformation of ImmCAFs to MyCAFs. (G) WNT pathway in ImmCAFs. (H) WNT pathway in MyCAFs. (I) Specific ligand activities of the WNT pathway in ImmCAFs. The WNT3A pathway was found to be involved in cellular communication in ImmCAFs. The ImmCAFs communicated with themselves and with epithelial cells. (J) Specific ligand activities of the WNT pathway in MyCAFs. The WNT3A and WNT2 pathways were found to be involved in cellular communication in MyCAFs. The MyCAFs communicated with themselves as well as with B, T/NK, endothelial, epithelial, MAST, and myeloid cells. GO gene ontology, CAFs cancer-associated fibroblasts, MyCAFs myofibroblastic cancer-associated fibroblasts, ImmCAFs immune-related cancer-associated fibroblasts, UMAP uniform manifold approximation and projection. P < 0.05 denotes statistically significant differences.