Fig. 4
From: Dusp1 in osteolytic diseases-mechanisms and therapeutic potential
Dusp1 overexpression modulated mRNA and protein expression in osteoclasts. (A-F) qPCR analysis of mRNA levels for Ctsk, Fos, Nfatc1, Mmp9, Acp5, and Atp6v0d2 in osteoclasts overexpressing Dusp1 or not. (G) Representative western blot images of NFATc1, c-Fos, and CTSK in osteoclasts overexpressing Dusp1 or not. To enhance clarity and conciseness, gels and blots were cropped. The original, uncropped images are provided in Supplementary Figure S8. The samples of each group were divided from the same experiment and were processed in parallel. (H-J) Quantification of NFATc1, c-Fos, and CTSK protein expression by gray value analysis relative to β-actin. Data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
