Table 2 Major components of eagle’s minimum essential medium (EMEM).
Group | Ingredients | Concentration | |
|---|---|---|---|
mg/L | mmol/L | ||
Inorganic salts | CaCl2 | 200.0 | 1.8 |
KCl | 400.0 | 0.8 | |
MgSO4 | 98.0 | 5.4 | |
NaCl | 6800.0 | 26.2 | |
NaHCO3 | 2200.0 | 116.4 | |
NaH2PO4 | 121.7 | 1.0 | |
BAA | L-Arginine·HCl | 126.0 | 0.6 |
L-Histidine·HCl·H2O | 42.0 | 0.2 | |
L-Lysine HCl | 72.5 | 0.4 | |
NAA | L-Cysteine | 23.8 | 2.0 |
L-Glutamine | 292.0 | 0.1 | |
L-Isoleucine | 52.0 | 0.4 | |
L-Leucine | 52.0 | 0.4 | |
L-Methionine | 15.0 | 0.1 | |
L-Phenylalanine | 32.0 | 0.2 | |
L-Threonine | 48.0 | 0.4 | |
L-Tryptophan | 10.0 | 0.0 | |
L-Tyrosine | 36.0 | 0.2 | |
L-Valine | 46.0 | 0.4 | |
Others | D-Glucose | 1000.0 | 5.55 |