Fig. 5 | Scientific Reports

Fig. 5

From: Volatile dimethyl disulphide emission from Burkholderia cepacia LS-044 suppresses metabolism and budding in caspofungin-resistant Nakaseomyces glabratus NT2

Fig. 5

Confocal microscopy revealing the impacts of the volatilome of Burkholderia cepacia LS-044 and standard dimethyl disulphide (DMDS) vapour on budding and cell wall peptidoglycans of Nakaseomyces glabratus NT2. Differential cellular morphology of control NT2 cells without exposure to bacterial volatiles (uppermost panel) and when exposed to the volatiles of LS-044 (middle panel) and the vapour of standard DMDS (lowermost panel) are shown (a). Cells were labeled with carboxyfluorescein succinimidyl ester (CFSE) and calcofluor white (CW) and subjected to excitation at green (491 nm) and blue (405 nm) lasers, respectively, and observed under a Zeiss confocal microscope. Budding cells, thickened luminous cell wall and scars are highlighted in red, yellow and white arrows, respectively. Scale bar, 5 μm. Differential fluorescence of yeast cells strained with CFSE when exposed to the volatiles of LS-044 and DMDS standard, as compared to control NT2 (without exposure to volatiles), is shown (b). Error bar, mean ± SD (n = 15). Statistical significance was determined using One-way ANOVA. ****p < 0.0001.

Back to article page