Fig. 2

The process of T-CS. A The abdominal cavity was opened under a sterile laminar flow hood to extract the FRT. B Clamping the base side of the cervix top. C Clamping the top of uterine horn. D Transferring cervix into isolator through connected trap with Clidox-S. E Opening the uterine horns with scissors and cleaning the pups with sterile cotton swab. F The pups were given to a GF foster mother when spontaneous breathing was noted.