Fig. 6
Characterization of transcriptome sequencing data between control and LMW-DFR-DM in keratinocytes and fibroblasts. (A) Venn diagram showing the number of genes shared and unique to each group (HaCaT, yellow; CCD-986sk, blue). (B, C) GO enrichment analysis of DEGs. GO enrichment terms in 562 DEGs clustered into 10 groups (upregulated in red, downregulated in green): apoptotic process (AP), Cell cycle (CC), Cell differentiation (CD), Cell migration (CM), Extracellular matrix (EM), inflammatory response (IR), aging (Ag.), autophagy (Au.), epidermis development (ED), and lysosome (Ly). (D) Scatter diagram of the DEGs. (E, F) Bar graphs of qRT-PCR indicating the ratio of related targeted gene expression in HaCaT and CCD-986sk cells (con-DFR-DM, blue; LMW-F-DFR-DM, red). Data are expressed as the mean ± SEM of triplicate assays, relative to con-DFR-DM. Statistical analysis was performed using Student’s t-test. **P < 0.01 and ***P < 0.001 versus corresponding controls.
