Fig. 1
Schematic overview of the experimental workflow. CLSM images were obtained from the in vitro INTERbACT model and in situ from splint-wearing volunteers. Following preprocessing, images were analyzed by three different methods, depending on image quality and content. Method 1 is a thresholding operation, which was applied for images without disturbing signals. For images with few disturbing signals, they were labeled in the Cellpose graphical user interface (GUI) and subtracted from the image before applying the thresholding operation (method 2). For method 3, two Cellpose models were used to segment the image separately. The resulting masks were combined and corrected in the Cellpose GUI (this figure was created using biorender.com).
