Fig. 1
From: A disinhibitory microcircuit in the temporal association cortex for fear retrieval to pure tones
Glutamatergic neurons in the TeA project to the TS. (A) Protocol for retrieval testing. CNO i.p. (B-D) The injection sites and expression of hM4D in TS with CNO i.p., and the freezing% during fear conditioning (C) and retrieval testing (D). hM4D- vs. hM4D+, t(16.16) = 12.79, p = 7.19 × 10−10 (D), t test for CS. (E) Injection of AAV2/retro-CAG-mCherry in TS (top) and expression(bottom). (F) Retrogradely labeled neurons projecting to TS in AuD, Au1, AuV and TeA. (G) Retrogradely labeled the TeATS neurons from rostral to caudal, located as the range from the bregma. (H) Distribution statistics. (I) Immunohistochemical staining of TeA. Blue: CaMKIIα; red: retrogradely labeled the TeATS neurons; purple: merge neurons. (J) Percentage of CaMKIIα-positive (merge) cell number in retrogradely labeled neurons (red).
