Fig. 7
From: A disinhibitory microcircuit in the temporal association cortex for fear retrieval to pure tones
Enhancement of ACh signaling in the TeA during auditory fear testing. (A) Injection and fiber photometry site (left) and expression (right) of AAV2/9-hSyn-GRABeen-ACh3.0 in unilateral TeA. (B) Heat maps (top) and mean Ach traces ± s.e.m. (shaded area) (bottom) of Ach signaling from TeA during preconditioning and retrieval test. (C) Statistics of CS-evoked freezing% (left) and ΔF/F% (right). Preconditioning vs. retrieval, t(7)=−6.89, p = 2.33 × 10−4, two-sided paired t test(left); t(7)=−10.54, p = 1.5 × 10−5, two-sided paired t test(right). (D) Injection of MCE and MLA into bilateral TeA before retrieval test. (E) Freezing during retrieval testing in mice of (D). CS: ACSF vs. MCE + MLA, t(17) = 13.97, p = 9.6 × 10−11, t test. (F) Injection (left) and expression (right) of AAV2/retro-CAG-eGFP in unilateral TeA. (G) An example showing TeA receiving projections from acetycholinergic neurons expressing ChAT (red) in BF.
