Fig. 4
From: Reference genes validation in tear fluid for RNA analysis in ocular surface disease

Stability ranking of candidate reference genes based on BestKeeper analysis. Each reference gene’s standard deviation (SD) values are plotted, with lower SD values indicating greater expression stability. Genes are ordered from the most stable on the left to the least stable on the right, highlighting AP3D1 as the most stable and UBC as the least stable reference gene. Control (n = 8), DED (n = 8), and glaucoma (n = 8) tear samples.