Table 3 Precision and accuracy results in brain homogenate.

From: Protoporphyrin IX plasma and blood pharmacokinetics and brain tumor distribution determined by a validated LC–MS/MS method

Analyte

QC ID

Nominal Conc. (nM)

Intra-batch (first batch)

Inter-batch

n

Mean Calculated conc. (nM)

Accuracy (%)

CV (%)

n

Mean Calculated conc. (nM)

Accuracy (%)

CV (%)

PPIX

LLOQ QC

1.00

5

0.951

95.1

5.9

15

1.02

102.0

10.7

LQC

3.00

5

2.85

94.9

2.5

15

2.84

94.6

4.3

MQC

60.0

5

54.7

91.2

0.8

15

57.9

96.4

6.5

HMQC

600

5

553

92.1

3.4

15

571

95.1

4.6

HQC

1500

5

1370

91.0

10.6

15

1450

96.6

7.5