Fig. 2 | Scientific Reports

Fig. 2

From: Toll like receptor 2 mediated exacerbation of sepsis associated acute kidney injury by renal congestion in mice

Fig. 2

Renal congestion exacerbated SA-AKI. (a) Experimental scheme and groups for analyses. (b) Serum TNFα measurements by ELISA in mice 1 day after CLP. (c) Representative pictures of Periodic Acid-Schiff (PAS), immunohistochemistry for F4/80, and dual immunofluorescence for KIM1 and LTL. (d) Semi-quantitative analysis of tubular injury by PAS staining. (e) qPCR of RNA from the whole kidney for representative markers of tubular injury (Havcr1 and Lcn2), tubular integrity (Lrp2), proinflammatory cytokines (Tnfα and Ccl2), macrophages (Cd68), and leukocytes (Ly6g). Bars indicate 50 μm in PAS and F4/80 staining and 100 μm in immunofluorescence staining in (c). In all groups, data are shown as means ± SD. N = 8 for uninjured mice and n = 12 for CLP mice. *P < 0.05. The paired t-test was used for comparisons of variables between CLK-CLP and Cong-CLP. ANOVA and Tukey’s post hoc test were used for comparisons of multiple variables.

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