Fig. 4
Renal congestion up-regulated the expression of toll-like receptors (TLR) and High Mobility Group Box 1 (HMGB1) in congestive kidneys with SA-AKI. (a) qPCR of RNA from the whole kidney for toll-like receptors (TLR)-2, -4, and -9 1 day after CLP (b) Representative immunofluorescence images of the kidney for TLR2 1 day after CLP. (c) Representative co-immunofluorescence images of the kidney for TLR2 and KIM1, PDGFRβ, and F4/80 1 day after CLP (d) Serum HMGB1 measurements by ELISA in mice 1 day after CLP (e) qPCR of RNA from the whole kidney for Hmgb1 1 day after CLP (f) Representative immunostaining images for HMGB1 1 day after CLP. The dotted square indicates high (*) and no (§) cytoplasmic HMGB1 expression in tubules. (g) Average HMGB1 scores in tubules. (h) Frequency of tubules in each score. Bar = 50 μm in (b) and (c) and = 30 μm in (f). In all groups, data are shown as means ± SD. The paired t-test was used for comparisons of variables between CLK-CLP and Cong-CLP. ANOVA and Tukey’s post hoc test were used for comparisons of multiple variables. * p < 0.05.
