Fig. 2

Smoothened (SMO) and Ki67 expression in periocular basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and sebaceous carcinoma (SEB). (A) SMO staining intensity was higher in all three tumor types than in the surrounding stroma (> 1 mm from the invasive front), but the values did not differ significantly between tumor types (Kruskal–Wallis, P = 0.03; Bonferroni-corrected significance threshold, P < 0.01). Post-hoc Dunn tests gave P > 0.99 for BCC vs. SCC and P = 0.08 for BCC vs. SEB. (B) The Ki67 index, quantified digitally in hot-spot areas with the highest proportion of Ki67-positive tumor nuclei, was likewise higher in all tumor types than in stroma, but showed no significant inter-tumor differences (Kruskal–Wallis, P > 0.39).