Fig. 1
Schematic map of SGI1ΔIn. SGI1ΔIn contains the entire conserved SGI1 backbone with an SmR/SpR cassette (aadA1) additionally inserted downstream of the helicase gene S023, but lacks the In104 cluster. Annotated ORFs S001-S044 are indicated by colour-coded arrows: green – recombination; orange – replication; blue – transcription regulator; yellow – T4SS components; purple – conjugation initiation, grey – TA system; red – antibiotic resistance gene; white – ORF of unknown function. Genes encoding hypothetical proteins are numbered according to the original numbering of SGI1 ORFs (e.g. ‘8’ refers to S008, etc.). Names of genes with known functions or identified homologs are marked accordingly with the following abbreviations: x – xis, C and D – sgaC and sgaD, B – mpsB. Colour-coded vertical lines indicate the relevant DNA elements: black – terminal direct repeats (DRL and DRR), green – origin of replication (oriV), blue – origin of transfer (oriT), purple – recombination (res) site obtained by the precise deletion of In104 with one copy of its flanking 5-bp DRs. The map is drawn to scale.
