Table 5 Ultra-high performance liquid chromatography mass spectrometer gradient elution mobile phase program settings.
Time | Flow | A. Conc | B. Conc | |
|---|---|---|---|---|
1 | 0 | 0.4 | 35.0 | 65.0 |
2 | 1.5 | 0.4 | 35.0 | 65.0 |
3 | 1.6 | 0.4 | 35.0 | 50.0 |
4 | 3.8 | 0.4 | 35.0 | 50.0 |
5 | 3.9 | 0.4 | 35.0 | 65.0 |
6 | 5.0 | 0.4 | 35.0 | 65.0 |