Fig. 1

Locomotor behavior in TMZ-treated zebrafish larvae. (A) Distance traveled (mm) and (B) velocity (mm/s) of zebrafish larvae pre- and post-TMZ administration at increasing TMZ concentrations. (C) Representative movement track pre- and post-TMZ (200µM) administration. n range = 34–84 larvae/group; specifically indicated in each panel. Statistical analysis was performed using the paired Wilcoxon test- Asterisks denote significance; ****p ≤ 0.0001). Data are presented as Mean ± SEM.