Fig. 3

Whole brain Ca²⁺ imaging upon TMZ exposure. (A) Representative z-projection images of the whole brain Ca²⁺ imaging performed in the rostral-dorsally placed Tg(neurod1:GCaMP6f) larvae before the treatment (PRE-TMZ) and after the treatment (POST-TMZ). On the right side, the look up table color range of Ca²⁺ fluorescence is shown. Images were taken with 4X magnification objective. The dotted line indicates the analyzed ROI. (B) A representative graph from a single zebrafish larva shows the ∆F/F0 Ca²⁺ fluorescent signal in the whole brain for each frame before and after TMZ treatment (C) The graph shows full distribution of data, with median and quartiles of ∆F/F0 fluorescent signal in whole brain. (D) The graph shows full distribution of data, with median and quartiles of frequency fluorescent signal in whole brain. n = 10 analyzed larvae. Since the same individuals were analyzed before and after the treatment, statistical analysis was performed using the paired Wilcoxon test. ns: not significant, (p of ∆F/F0 = 0.19; p of frequency = 0.06;).