Fig. 3 | Scientific Reports

Fig. 3

From: Effect of defective H2B ubiquitination on the malignancy and repair of DNA double breaks and its significance in lung adenocarcinoma

Fig. 3

Effects of RNF20 on A549 Cell Migration and Proliferation. (a) Transfection of A549 cells with RNF20 shRNA or control shRNA vectors. (b) Western blot analysis of RNF20 and uH2B in control and RNF20 shRNA-transfected A549 cells. (c) The CCK-8 assay analysis of cell proliferation in the RNF20-deficient group compared to controls at 24 h, 48 h, and 72 h. Western blot analysis was performed to assess time-dependent variations in RNF20 protein levels post-silencing and to validate the stable knockdown efficiency of RNF20. (d) Scratch assay showing migration in RNF20-silenced A549 cells, magnification: 4×. (**P < 0.01, ***P < 0.001)

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