Fig. 2
From: Matrix metalloproteinase-2 as a novel regulator of glucose utilization by adipocytes
MMP2 decreases glycolysis in 3T3-L1 adipocytes. (A) Aerobic glycolysis of adipocytes after treatment with 400 nM insulin without or with 200 ng/mL, 400 ng/mL, or 800 ng/mL of active MMP2 (ECAR, extracellular acidification rate). (B) MMP substrate cleavage assay: MMP FRET Substrate which becomes fluorescent after cleavage, was incubated with 400 ng/ml (5 nM) MMP2, MMP2 inhibitor I only (1 µM), MMP2 with MMP2 inhibitor I, or MMP2 with 50 mM EDTA. (C) Aerobic glycolysis of adipocytes after treatment with insulin with 400 ng/mL (5 nM) of active MMP2, MMP2 inhibitor I (1 µM), or MMP2 with MMP2 inhibitor I. Values are expressed as means + SEM. *, p < 0.05; **, p < 0.01; ***, p > 0.001, ****, p < 0.0001 two-way ANOVA with Tukey’s multiple comparison test. n = 4 from single experiment (A,C) and n = 6 (B).
