Fig. 7
The effect of MTF silencing on target gene expression, as determined using in vitro analyses. 59 M cells were treated with control siRNA (siControl) or three MTF siRNAs for 48 h. q-PCR results showing the expression of MTFs (MSC, AEBP1, and CREB3L1) (A and C), mRNA target genes (D and F), and lncRNAs (G and I) in transfected cells are shown. Data are presented as the mean ± S.E.M. P-values were calculated using the Mann–Whitney U test, and all experiments were performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Statistically insignificant results are not shown. Abbreviations: MSC, Musculin; AEBP1, AE binding protein 1; CREB3L1, CAMP responsive element binding protein 3 like 1; COL3A1, Collagen type III alpha 1 chain; LUM, Lumican; DCN, Decorin; FNDC1, Fibronectin type III domain containing 1; THBS2, Thrombospondin-2; COL1A1, Collagen, type I, alpha 1; MMP2, matrix metalloproteinase-2; FN1, Fibronectin 1; FAP, fibroblast activation protein alpha; LINC01614, Long intergenic non-protein coding RNA 1614; LINC00702, Long intergenic non-protein coding RNA 702; LINC02544, Long intergenic non-protein coding RNA 2544; AL356417.2, novel transcript, antisense to FNDC1; AC112721.2, novel protein (LOC728009); LINC01929, Long intergenic non-protein coding RNA 1929.
