Fig. 1

Identification of the pupal stage when each subtype is produced from neuroblasts. (A) A scheme of the experimental design. Last instar larvae were collected from the bee hives and EdU was injected into the pupal heads at various pupal stages. After emergence, EdU detection and ISH in the brain sections were performed. Schematic diagram of a honey bee MB (the region filled with blue in the brain schematic) is shown on the right. (B-D) Distribution of EdU signals in the brain of adult individuals injected with EdU at 14hap (B), 30hap (C), and 85hap (D). From left to right panels, the EdU signals, jhdk FISH signals, merged signals, and schematic diagrams of EdU signals and each subtype are shown. Ca, a calyx in the MB. Scale bar; 100 μm. (E) Correspondence between the pupal stage injected with EdU and the subtypes where EdU signals were detected. The size of each circle represents the approximate proportion of EdU-detected cells in each subtype.